Principals and Techiniques of Biochemistry and Molecular Biology 7th Edition

Principals and Techiniques of Biochemistry and Molecular Biology

PDF Free Download | Principals and Techiniques of Biochemistry and Molecular Biology 7th Edition by Keith Wilson and John Walker

Contents of Biochemistry and Molecular Biology

  • Basic principles
  • Biochemical and molecular biology studies
  • Units of measurement
  • Weak electrolytes
  • Quantitative biochemical measurements
  • Safety in the laboratory
  • Suggestions for further reading
  • Cell culture techniques
  • The cell culture laboratory and equipment
  • Safety considerations in cell culture
  • Aseptic techniques and good cell culture practice
  • Types of animal cell, characteristics and maintenance in culture
  • Stem cell culture
  • Bacterial cell culture
  • Potential use of cell cultures
  • Suggestions for further reading
  • Centrifugation
  • Introduction
  • Basic principles of sedimentation
  • Types, care and safety aspects of centrifuges
  • Preparative centrifugation
  • Analytical centrifugation
  • Suggestions for further reading
  • Microscopy
  • The light microscope
  • Optical sectioning
  • Imaging living cells and tissues
  • Measuring cellular dynamics
  • The electron microscope (EM)
  • Image archiving
  • Suggestions for further reading
  • Molecular biology, bioinformatics and basic techniques
  • Structure of nucleic acids
  • Genes and genome complexity
  • Location and packaging of nucleic acids
  • Functions of nucleic acids
  • The manipulation of nucleic acids – basic tools and techniques
  • Isolation and separation of nucleic acids
  • Molecular biology and bioinformatics
  • Molecular analysis of nucleic acid sequences
  • The polymerase chain reaction (PCR)
  • Nucleotide sequencing of DNA
  • Suggestions for further reading
  • Recombinant DNA and genetic analysis
  • Introduction
  • Constructing gene libraries
  • Cloning vectors
  • Hybridisation and gene probes
  • Screening gene libraries
  • Applications of gene cloning
  • Expression of foreign genes
  • Analysing genes and gene expression
  • Analysing whole genomes
  • Pharmacogenomics
  • Molecular biotechnology and applications
  • Suggestions for further reading
  • Immunochemical techniques
  • Making antibodies
  • Immunoassay formats
  • Immuno microscopy
  • Lateral flow devices
  • Epitope mapping
  • Immunoblotting
  • Fluorescent activated cell sorting (FACS)
  • Cell and tissue staining techniques
  • Immunocapture polymerase chain reaction (PCR)
  • Immunoaffinity chromatography (IAC)
  • Antibody-based biosensors
  • Therapeutic antibodies
  • The future uses of antibody technology
  • Suggestions for further reading
  • Protein structure, purification, characterisation
  • and function analysis
  • Ionic properties of amino acids and proteins
  • Protein structure
  • Protein purification
  • Protein structure determination
  • Proteomics and protein function
  • Suggestions for further reading
  • Mass spectrometric techniques
  • Ionisation
  • Mass analysers
  • Detectors
  • Structural information by tandem mass spectrometry
  • Analysing protein complexes
  • Computing and database analysis
  • Suggestions for further reading
  • Electrophoretic techniques
  • General principles
  • Support media
  • Electrophoresis of proteins
  • Electrophoresis of nucleic acids
  • Capillary electrophoresis
  • Microchip electrophoresis
  • Suggestions for further reading
  • Chromatographic techniques
  • Principles of chromatography
  • Chromatographic performance parameters
  • High-performance liquid chromatography
  • Adsorption chromatography
  • Partition chromatography
  • Ion-exchange chromatography
  • Molecular (size) exclusion chromatography
  • Affinity chromatography
  • Gas chromatography
  • Suggestions for further reading
  • Spectroscopic techniques: I Spectrophotometric techniques
  • Introduction
  • Ultraviolet and visible light spectroscopy
  • Fluorescence spectroscopy
  • Luminometry
  • Circular dichroism spectroscopy
  • Light scattering
  • Atomic spectroscopy
  • Suggestions for further reading
  • Spectroscopic techniques: II Structure and interactions
  • Introduction
  • Infrared and Raman spectroscopy
  • Surface plasmon resonance
  • Electron paramagnetic resonance
  • Nuclear magnetic resonance
  • X-ray diffraction
  • Small-angle scattering
  • Suggestions for further reading
  • Radioisotope techniques
  • Why use a radioisotope?
  • The nature of radioactivity
  • Detection and measurement of radioactivity
  • Other practical aspects of counting of radioactivity and analysis of data
  • Safety aspects
  • Suggestions for further reading
  • Enzymes
  • Characteristics and nomenclature
  • Enzyme steady-state kinetics
  • Analytical methods for the study of enzyme reactions
  • Enzyme active sites and catalytic mechanisms
  • Control of enzyme activity
  • Suggestions for further reading
  • Principles of clinical biochemistry
  • Principles of clinical biochemical analysis
  • Clinical measurements and quality control
  • Examples of biochemical aids to clinical diagnosis
  • Suggestions for further reading
  • Cell membrane receptors and cell signalling
  • Receptors for cell signalling
  • Quantitative aspects of receptor–ligand binding
  • Ligand-binding and cell-signalling studies
  • Mechanisms of signal transduction
  • Receptor trafficking
  • Suggestions for further reading
  • Drug discovery and development
  • Human disease and drug therapy
  • Drug discovery
  • Drug development
  • Suggestions for further reading
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