Principals and Techiniques of Biochemistry and Molecular Biology 7th Edition by Keith Wilson and John Walker
Contents of Biochemistry and Molecular Biology
- Basic principles
- Biochemical and molecular biology studies
- Units of measurement
- Weak electrolytes
- Quantitative biochemical measurements
- Safety in the laboratory
- Suggestions for further reading
- Cell culture techniques
- The cell culture laboratory and equipment
- Safety considerations in cell culture
- Aseptic techniques and good cell culture practice
- Types of animal cell, characteristics and maintenance in culture
- Stem cell culture
- Bacterial cell culture
- Potential use of cell cultures
- Suggestions for further reading
- Centrifugation
- Introduction
- Basic principles of sedimentation
- Types, care and safety aspects of centrifuges
- Preparative centrifugation
- Analytical centrifugation
- Suggestions for further reading
- Microscopy
- The light microscope
- Optical sectioning
- Imaging living cells and tissues
- Measuring cellular dynamics
- The electron microscope (EM)
- Image archiving
- Suggestions for further reading
- Molecular biology, bioinformatics and basic techniques
- Structure of nucleic acids
- Genes and genome complexity
- Location and packaging of nucleic acids
- Functions of nucleic acids
- The manipulation of nucleic acids – basic tools and techniques
- Isolation and separation of nucleic acids
- Molecular biology and bioinformatics
- Molecular analysis of nucleic acid sequences
- The polymerase chain reaction (PCR)
- Nucleotide sequencing of DNA
- Suggestions for further reading
- Recombinant DNA and genetic analysis
- Introduction
- Constructing gene libraries
- Cloning vectors
- Hybridisation and gene probes
- Screening gene libraries
- Applications of gene cloning
- Expression of foreign genes
- Analysing genes and gene expression
- Analysing whole genomes
- Pharmacogenomics
- Molecular biotechnology and applications
- Suggestions for further reading
- Immunochemical techniques
- Making antibodies
- Immunoassay formats
- Immuno microscopy
- Lateral flow devices
- Epitope mapping
- Immunoblotting
- Fluorescent activated cell sorting (FACS)
- Cell and tissue staining techniques
- Immunocapture polymerase chain reaction (PCR)
- Immunoaffinity chromatography (IAC)
- Antibody-based biosensors
- Therapeutic antibodies
- The future uses of antibody technology
- Suggestions for further reading
- Protein structure, purification, characterisation
- and function analysis
- Ionic properties of amino acids and proteins
- Protein structure
- Protein purification
- Protein structure determination
- Proteomics and protein function
- Suggestions for further reading
- Mass spectrometric techniques
- Ionisation
- Mass analysers
- Detectors
- Structural information by tandem mass spectrometry
- Analysing protein complexes
- Computing and database analysis
- Suggestions for further reading
- Electrophoretic techniques
- General principles
- Support media
- Electrophoresis of proteins
- Electrophoresis of nucleic acids
- Capillary electrophoresis
- Microchip electrophoresis
- Suggestions for further reading
- Chromatographic techniques
- Principles of chromatography
- Chromatographic performance parameters
- High-performance liquid chromatography
- Adsorption chromatography
- Partition chromatography
- Ion-exchange chromatography
- Molecular (size) exclusion chromatography
- Affinity chromatography
- Gas chromatography
- Suggestions for further reading
- Spectroscopic techniques: I Spectrophotometric techniques
- Introduction
- Ultraviolet and visible light spectroscopy
- Fluorescence spectroscopy
- Luminometry
- Circular dichroism spectroscopy
- Light scattering
- Atomic spectroscopy
- Suggestions for further reading
- Spectroscopic techniques: II Structure and interactions
- Introduction
- Infrared and Raman spectroscopy
- Surface plasmon resonance
- Electron paramagnetic resonance
- Nuclear magnetic resonance
- X-ray diffraction
- Small-angle scattering
- Suggestions for further reading
- Radioisotope techniques
- Why use a radioisotope?
- The nature of radioactivity
- Detection and measurement of radioactivity
- Other practical aspects of counting of radioactivity and analysis of data
- Safety aspects
- Suggestions for further reading
- Enzymes
- Characteristics and nomenclature
- Enzyme steady-state kinetics
- Analytical methods for the study of enzyme reactions
- Enzyme active sites and catalytic mechanisms
- Control of enzyme activity
- Suggestions for further reading
- Principles of clinical biochemistry
- Principles of clinical biochemical analysis
- Clinical measurements and quality control
- Examples of biochemical aids to clinical diagnosis
- Suggestions for further reading
- Cell membrane receptors and cell signalling
- Receptors for cell signalling
- Quantitative aspects of receptor–ligand binding
- Ligand-binding and cell-signalling studies
- Mechanisms of signal transduction
- Receptor trafficking
- Suggestions for further reading
- Drug discovery and development
- Human disease and drug therapy
- Drug discovery
- Drug development
- Suggestions for further reading
